Effect of Pro197Leu Polymorphism of the Gene GPX1 on Carbohydrate Metabolism and Anthropometric Indices of Patients with Arterial Hypertension Against the Background of Abdominal Obesity

N.O. ABRAMOVA, N.V. PASHKOVSKA

Abstract


The aim of our study was to investigate the dependence of carbohydrate metabolism in patients with arterial hypertension and concomitant abdominal obesity depending on Pro197Leu polymorphism of the GPX1 gene. Pro197Leu polymorphism of the gene GPX1 in 102 patients with arterial hypertension and concomitant abdominal obesity and 97 healthy individuals have been studied. Disorders of distribution of genotype frequencies comparing with the control group on account of the reduction of Pro/Pro genotype frequency have been found in the main group. Analyzing the data, the growth of risk of disorder in the GPX1 activity in patients with Pro/Leu and Leu/Leu variants of polymorphism comparing with homozygotes for the wild allele at 4.7 and 6.9 times, respectively had been revealed. Analyzing changes of carbohydrate metabolism depending on the Pro197Leu polymorphism of the gene GPX1, it was established that in patients with Leu/Leu genotype the production of immunoreactive insulin, leptin, C-peptide increased significantly, HOMA-IR and BMI was significantly higher compared with the persons with Pro/Pro genotype. Thus, Pro-allele possesses protective properties as to the reduction in the activity of glutathione peroxidase. Insulin and leptin resistance develop in the carriers of Leu-allele, which causes disturbances in carbohydrate metabolism.

Keywords


Pro197Leu polymorphism of the gene GPX1; carbohydrate metabolism; insulin resistance; metabolic syndrome

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References


Жейкова Т.В. Генетическая основа регуляции окислительного стресса: связь с продолжительностью жизни и ишемической болезнью сердца: Автореф. дис... канд. мед. наук: 03.02.07. — Томск, 2013. — 24 с.

Харченко Н.В. Метаболический синдром в практике гастроэнтеролога // Здоров’я України. — 2008. — № 19 (1). — С. 5-6.

Bastaki M. Genotype-activity relationship for Mn-superoxide dismutase, glutathione peroxidase 1 and catalase in humans / M. Bastaki, K. Huen, P. Manzanillo // Pharmacogenet. Genomics. — 2006. — № 16. — P. 279-286.

Brosnan M.J. One step beyond glutathione peroxidase and endothelial dysfunction // Hypertension. — 2008. — № 51. — P. 825-826.

De Oliveira Hiragi C., Miranda-Vilela A.L., Rocha D.M. et al. Superoxide dismutase, catalase, glutathione peroxidase and gluthatione S-transferases M1 and T1 gene polymorphisms in three Brazilian population groups // Genet. Mol. Biol. — 2011. — Vol. 34, № 1. — P. 11-18.

Crawford A. Glutathione peroxidase, superoxide dismutase and catalase genotypes and activities and the progression of chronic kidney disease / A. Crawford, R. Fassett, G. Robert [et al.] // Nephrology, Dialysis, Transplantation. — 2011. — Vol. 26, № 9. — P. 2806-2813.

Fabre E.E. Gene polymorphisms of oxidative stress enzymes: prediction of elderly renutrition / Fabre E.E., Raynaud-Simon A., Golmard J.-L. [et al.] // Am. J. Clin. Nutr. — 2008. — Vol. 87, № 5. — P. 1504-1512.

Hu Y. Allelic Loss of the Gene for the GPX1 Selenium-Containing Protein Is a Common Event in Cancer / Y. Hu, R.V. Benya, R.E. Carroll // J. Nutr. — 2005. — Vol. 135, № 12. — P. 3021-3024.

Kobayashi H. Dysregulated glutathione metabolism links to impaired insulin action in adipocytes / H. Kobayashi, M. Matsuda, A. Fukuhara [et al.] // American Journal of Physiology — Endocrinology and Metabolism. — 2009. — Vol. 296. — P. 1326-1334.

Miranda-Vilela A.L. Gene polymorphisms against DNA damage induced by hydrogen peroxide in leukocytes of healthy humans through comet assay: a quasi-experimental study / Miranda-Vilela A.L., Alves P.C., Akimoto A.K. // Environmental Health. — 2010. — Vol. 9, № 21. — Режим доступa: http://

www.ehjournal.net/content/9/1/21

Nemoto M. Genetic association of glutathione peroxidase 1 gene with coronary artery calcification in type 2 diabetes: a case control study with multi-slice computed tomography / M. Nemoto, R. Nishimura, T. Sasaki [et al.] // Cardiovascular Diabetology. — 2007. — Vol. 6, № 23. — Режим доступа: http://www.cardiab.com/content/6/1/23.

Жейкова Т.В., Голубенко М.В., Буйкин С.В. и др. Полиморфизм Pro198 → Leu гена глутатионпероксидазы (GPX1): связь с продолжительностью жизни и ишемической болезнью сердца // Молекулярная биология. — 2012. — Т. 46, № 3. — С. 481-486.




DOI: https://doi.org/10.22141/2224-0721.6.54.2013.84626

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