The State of Microbiota of the Large Intestine in Patients with Type 1 Diabetes Mellitus Depending on the Age and Duration of the Disease

О.О. Mazur, О.H. Plaksyvyi, N.V. Pashkovska, І.О. Bilooka


Introduction. Intestinal dysbiosis against the background of type 1 diabetes mellitus (DM) results in the development and unfavourable clinical course of chronic diseases, in particular of ENT-organs. Objective of the study — to investigate the species composition and population level of microflora of the large intestine in patients with DM type 1 depending on the disease duration and age of patients. Material and methods. With the use of modern bacteriological and mycological methods, we have studied species composition and population level of the microbiota of the large intestine in 50 patients with type 1 DM of the following age groups: 15–20 years (n = 10); 21–35 years (n = 14); 36–60 years (n = 26). Results. In patients with DM type 1, there occurs a significant deficiency of autochthonous anaerobic bifido- and lactobacteria in the contents of the large intestine. With the age of the patient, this deficiency increases, a clear picture of reducing the role of bifidobacteria in the large intestine microbiocenosis is being predicted. A similar situation is emerging in bacteria Lactobacillus, Peptostreptococcus and Enterococcus. There is an increase of the biological role in the microbiocenosis of bacteroides, pathogenic (E.coli Hly+, enteropathogenic E.coli) and opportunistic (Proteus, Enterobacter, Citrobacter) enterobacteria, clostridia, staphylococci and yeast-like fungi of the genus Candida. Conclusions. With age, there is an increase in the negative processes of changes in species composition and population level, constancy index, Berger-Parker dominance, detection rate, coefficient of quantification dominance and importance. There occurs a deficiency and reduction in the role in microbiocenosis of bacteria of the genus Bifidobacterium, Lactobacillus, Peptostreptococcus and bacteria of the genus Enterococcus.


type 1 diabetes mellitus; normal microflora; large intestine; dysbiosis.


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