Main Article Content
Background. The purpose: to carry out a comparative analysis of the cytotoxic activity of natural killer (NK) cells and the metabolic activity of neutrophils before and after radioiodine therapy (RIT) in patients with differentiated thyroid cancer depending on gender, age, the presence of distant metastases and in persons with toxic goiter (toxic goiter) depending on gender. Materials and methods. Immunological parameters were evaluated in three groups of thyroidectomized patients with thyroid cancer. Group 1 included 48 people (30 women, 18 men) of young age (from 11 to 39 years) without distant metastases of thyroid cancer. Group 2 consisted of 40 patients (23 women, 17 men) of young age (from 16 to 40 years) with distant metastases of thyroid cancer. Group 3 consisted of 55 patients (30 women, 25 men) of older age (from 41 to 75 years) with distant metastases of thyroid cancer. The average number of RIT courses in women of group 1 was 1.10 ± 0.04, in men — 1.50 ± 0.24, in women of group 2 — 5.4 ± 0.7, in men — 6.3 ± 0.8. In older patients (group 3), the average number of RIT courses was 5.4 ± 0.3 in women, and 5.5 ± 0.7 in men. Immunological parameters were evaluated in 30 patients (22 women, 8 men) with toxic goiter aged 23 to 67 years. Patients with toxic goiter received a course of RIT. Immunological study was carried out the day before RIT and 5 or 6 days after it. The cytotoxic activity of NK cells against indicator target cells was determined using spectrophotometry. The metabolic activity of neutrophils was studied in a standard test for the reduction of nitroblue tetrazolium. Results. In the early stages after RIT, the activity of NK cells decreased in young men of group 1. In young men of group 2, the activity of NK cells after RIT decreased to a greater extent than in group 1. The activity of NK cells in older men of group 3 both before and after RIT was significantly lower than that of women and significantly lower than that of young men. After RIT, the metabolic activity of neutrophils increased in men and women with thyroid cancer in group 1 and in men of group 2. In women of group 1, this indicator was significantly higher than in men. In men and women of group 2, the metabolic activity of neutrophils after RIT was significantly lower than in patients of group 1. In male patients with toxic goiter, the activity of NK cells the day before RIT was lower, and the metabolic activity of neutrophils was much higher than in women. After RIT, an increase in the activity of NK cells in toxic goiter patients occurs to a greater extent in females, and in the metabolic activity of neutrophils — in males. Conclusions. A comparative analysis of the results of studying the early effect of 131I on the cytotoxic activity of NK cells and the metabolic activity of neutrophils revealed the peculiarities of changes in these parameters in patients with thyroid cancer depending on age, gender, the presence of distant metastases, and in patients with toxic goiter — depending on gender.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our edition uses the copyright terms of Creative Commons for open access journals.
Authors, who are published in this journal, agree with the following terms:
- The authors retain rights for authorship of their article and grant to the edition the right of first publication of the article on a Creative Commons Attribution 4.0 International License, which allows others to freely distribute the published article, with the obligatory reference to the authors of original works and original publication in this journal.
- Directing the article for the publication to the editorial board (publisher), the author agrees with transmitting of rights for the protection and using the article, including parts of the article, which are protected by the copyrights, such as the author’s photo, pictures, charts, tables, etc., including the reproduction in the media and the Internet; for distributing; for the translation of the manuscript in all languages; for export and import of the publications copies of the writers’ article to spread, bringing to the general information.
- The rights mentioned above authors transfer to the edition (publisher) for the unlimited period of validity and on the territory of all countries of the world.
- The authors guarantee that they have exclusive rights for using of the article, which they have sent to the edition (publisher). The edition (the publisher) is not responsible for the violation of given guarantees by the authors to the third parties.
- The authors have the right to conclude separate supplement agreements that relate to non-exclusive distribution of their article in the form in which it had been published in the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.
- The policy of the journal permits and encourages the publication of the article in the Internet (in institutional repository or on a personal website) by the authors, because it contributes to productive scientific discussion and a positive effect on efficiency and dynamics of the citation of the article.
Tkachenko VI, Rimar YaYu. Morbidity and prevalence of malignant neoplasms of the thyroid gland in Ukraine and Kyiv region after the Chernobyl Nuclear Power Plant accident. Mìžnarodnij endokrinologìčnij žurnal. 2019;15(2):152-157. doi: 10.22141/2224-07220.127.116.119.166109.
Kitahara CM, K Rmendiné Farkas D, Jørgensen JOL, Cronin-Fenton D2, Sørensen HT. Benign Thyroid Diseases and Risk of Thyroid Cancer: A Nationwide Cohort Study. J Clin Endocrinol Metab. 2018;103(6):2216-2224. doi: 10.1210/jc.2017-02599.
Cheng F, Chen Y, Zhu L, et al. Risk Factors for Cervical Lymph Node Metastasis of Papillary Thyroid Microcarcinoma: A Single-Center Retrospective Study. Int J Endocrinol. 2019;2019:8579828. doi: 10.1155/2019/8579828.
Kim MJ, Lee SG, Kim K. et al. Current trends in the features of male thyroid cancer: Retrospective evaluation of their prognostic value. Medicine (Baltimore). 2019;98(19):e15559. doi: 10.1097/MD.0000000000015559.
Khmara IM. Immunity indices and their dynamics in patients with differentiated forms of thyroid cancer in the course of complex medical rehabilitation. Mediko-biologicheskie problemy zhiznedeiatel'nosti. 2009;1:112-120. (in Russian).
Li C, Lee KC, Schneider EB, Zeiger MA. BRAF V600E mutation and its association with clinicopathological features of papillary thyroid cancer: a meta-analysis. J Clin Endocrinol Metab. 2012;97(12):4559-70. doi: 10.1210/jc.2012-2104.
Zakharchenko TF, Zamotayeva GA, Gulevaty SV, Volynets IP, Dzhuzha DO. Dynamics of indicators activity of innate immunity in patients with distant metastases differentiated thyroid carcinoma of different age groups after radioiodine therapy. Problems of Endocrine Pathology. 2015;2:35-41. (in Ukrainian).
Gogali F, Pateracis G, Rassidakis GZ. et al. Phenotypical analysis of lymphocytes with suppressive and regulatory properties (tregs) and NK cells in papillary carcinoma of thyroid. J Clin Endocrinol Metab. 2012 May;97(5):1474-82. doi: 10.1210/jc.2011-1838.
Tofani A, Sciuto R, Cioffi RP, et al. Radioiodine-induced changes in lymphocyte subsets in patients with differentiated thyroid carcinoma. Eur J Nucl Med. 1999 Aug;26(8):824-9. doi: 10.1007/s002590050455.
Luchytsky VYe, Luchytsky YeV, Zubkova GA, et al. Levels of gonadotropic and sexual hormones in men with thyroid cancer in the dynamics of treatment. Endokrynologia. 2016;21(2):125-130. (in Ukrainian).
Faria CC, Peixoto MS, Carvalho DP, Fortunato RS. The emerging role of estrogens in thyroid redox homeostasis and carcinogenesis. Oxid Med Cell Longev. 2019 Jan 9;2019:2514312. doi: 10.1155/2019/2514312.
Sato S, Takahashi I, Komame H, et al. Association of sex steroid hormones with neutrophil function in the general population. Hirosaki Med J. 2016;67:13-27.
Rosário PW, Batista KC, Calsolari MR. Radioiodine-induced oxidative stress in patients with differentiated thyroid carcinoma and effect of supplementation with vitamins C and E and selenium (antioxidants). Arch Endocrinol Metab. 2016;60(4):328-32. doi: 10.1590/2359-3997000000128.
Zhang Y, Lv G, Lou X. et al. NKG2A expression and impaired function of NK cells in patients with new onset of Graves’ disease. Int Immunopharmacol. 2015;24(1):133-9. doi: 10.1016/j.intimp.2014.09.020.
Zakharchenko TF, Gulevaty SV, Volynets IP. Comparative analysis of the activity of cells of innate immunity in patients with benign and malignant disorders of thyroid glands after radiodine therapy. Mìžnarodnij endokrinologìčnij žurnal. 2019;15(3):210-216. doi: 10.22141/2224-0718.104.22.1689.172106. (in Ukrainian).
Polanczyk MJ, Carson BD, Subramanian S, et al. Cutting edge: estrogen drives expansion of the CD4+CD25+ regulatory T cell compartment. J Immunol. 2004;173(4):2227-30. doi: 10.4049/jimmunol.173.4.2227.
Curran EM, Berghaus LJ, Vernetti NJ, Saporita AJ, Lubahn DB, Estes DM. Natural killer cells express estrogen receptor-alpha and estrogen receptor-beta and can respond to estrogen via a non-estrogen receptor-alpha-mediated pathway. Cell Immunol. 2001;214(1):12-20. doi: 10.1006/cimm.2002.1886.
Côté-Bigras S, Tran V, Turcotte S, Rola-Pleszczynski M, Verreault J, Rottembourg D. Impaired immune regulation after radioiodine therapy for Graves’ disease and the protective effect of Methimazole. Endocrine. 2016 Jun;52(3):587-96. doi: 10.1007/s12020-015-0832-2.
Wenzel BE, Chow A, Baur R, Schleusener H, Wall JR. Natural killer cell activity in patients with Graves’ disease and Hashimoto’s thyroiditis. Thyroid. 1998;8(11):1019-1022. doi: 10.1089/thy.1998.8.1019.
Dogadin SA, Dudina MA, Savchenko AA. Respiratory burst activity in neutrophilic granulocytes in the onset of Graves’ disease. Problems of Endocrinology. 2017;63(1):4-8. doi: 10.14341/probl20176314-8. (in Russian).
Owczarek T, Kowalczyk E, Poliwczak AR, Bała A, Broncel M. The evaluation of selected oxidative stress parameters in patients after radioiodine treatment of hyperthyroidism. Pol Merkur Lekarski. 2012;32(192):382-7.