Comparative characteristics of proteolytic activity in case of experimental peritonitis and its development on the background of diabetes mellitus
Background. The relevance is due to rather understudied state of proteolytic reactions in case of diabetes mellitus (DM) with acute peritonitis (AP), which is increasingly common in the practice. Objective: to study the features of proteolytic activity of plasma in AP associated with DM. Materials and methods. One hundred albino outbred rats. AP was simulated by the transesophageal perforation of the stomach. DM was simulated by the 1.6% alloxan solution injection. The proteolytic activity of blood plasma was studied by azocasein (AzCs), azoalbumin (AzAl), and azocollagen (AzCl). The animals were divided into the following groups: intact rats, animals with simulated DM, intact rats with simulated peritonitis (group 1), animals with models of peritonitis on the background of DM (group 2). Results. The initial level of proteolytic transformation of AzCs and AzAl in animals with simulated DM was significantly higher. The proteolytic transformation level of AzCl had almost no differences. Six hours after the moment AP was modeled, the proteolytic transformation level of AzCs increased in both groups, more significantly in group 1, although this indicator in group 1 remained less. The proteolytic transformation level of AzAl increased significantly. The proteolytic transformation level of AzCl in group 1 remained almost the same, and increased significantly in group 2. In 12 hours, the proteolytic transformation level of AzCs decreased slightly in group 1 but continued to increase in group 2. The proteolytic transformation level of AzAl significantly increased in both groups, and the indicators in group 2 were higher. The proteolytic transformation level of AzCl decreased statistically significantly in group 1, and greatly increased in group 2. In 24 hours, the proteolytic transformation level of AzCs had almost no changes in group 1 but continued to increase in group 2. The proteolytic transformation level of AzAl decreased significantly in group 1 and greatly increased in group 2. The proteolytic transformation level of AzCl significantly increased in both groups. In 48 hours, the proteolytic transformation level of AzCs and AzAl increased in both groups but the indicators in group 2 were higher. The proteolytic transformation level of AzCl decreased slightly in group 1, and continued to increase in group 2. Conclusions. The proteolytic transformation activity of high and low molecular weight blood plasma proteins increases in experimental diabetes mellitus. The proteolytic system of plasma is activated, with maintaining the balance between its links within 24 hours in experimental acute peritonitis. The development of acute peritonitis in animals with simulated diabetes mellitus differs greatly in 6 hours by quantitative characteristics of the proteolytic activity of blood plasma that manifested with its significant increase, some imbalance between the links of proteolysis with the signs of uncontrolled proteolysis in 24 hours. The differences being detected are due to the changes in the functional activity of the proteolytic system caused by diabetes mellitus that underlies the disorders of the mechanisms of inflammation regulation.
Yaribeygi H, Atkin SL, Ramezani M, Sahebkar A. A review of the molecular pathways mediating the improvement in diabetes mellitus following caloric restriction. J Cell Physiol. 2019 Jun;234(6):8436-8442. doi: 10.1002/jcp.27760.
Grynchuk FV, Grynchuk AF, Maksimiuk VV. Comparative characteristic of fibrinolytic activity in case of experimental peritonitis and underlying diabetes mellitus. Mìžnarodnij endokrinologìčnij žurnal. 2017;13(8):618-623. doi: 10.22141/2224-07220.127.116.117.119281.
Yaribeygi H, Atkin SL, Sahebkar A. Mitochondrial dysfunction in diabetes and the regulatory roles of antidiabetic agents on the mitochondrial function. J Cell Physiol. 2019 Jun;234(6):8402-8410. doi: 10.1002/jcp.27754.
Swe MT, Pongchaidecha A, Chatsudthipong V, Chattipakorn N, Lungkaphin A. Molecular signaling mechanisms of renal gluconeogenesis in nondiabetic and diabetic conditions. J Cell Physiol. 2019 Jun;234(6):8134-8151. doi: 10.1002/jcp.27598.
Kuharchuk OL.Patogenetychna rol' ta metody korekcii' integratyvnyh porushen' gormonal'no-mesendzhernyh system reguljacii' gomeostazu natriju pry patologii' nyrok. Diss. dokt. med. nauk [Pathogenetic role and correction methods of integrative disorders of hormonal-messenger systems of regulation of sodium homeostasis in renal pathology. Dr. med. sci. diss.]. Odesa; 1996. 36 p. (in Ukrainian).
Veremeenko KN, Goloborod’ko OP, Kizim AI. Proteoliz v norme i pri patologi [Proteolysis in normal and pathological conditions]. Kyiv: Zdorov’ja; 1988. 200 p. (in Russian).
Colman RW, Hirsh J, Marder VJ, et al., editors. Hemostasis and thrombosis: basic principles and clinical practice. 4th ed. Philadelphia: Lippincott Williams and Wilkins; 2001. 1578 p.
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