Influence of recombinant human thyroid-stimulating hormone of and radioiodine therapy on the function of cells of innate immunity in patients with thyroid cancer

T.F. Zakharchenko, G.A. Zamotaeva, S.V. Gulevatyi


Background. The effect of radioiodine therapy (RIT) on the cytotoxic activity of NK cells and the metabolic activity of neutrophils (NF) in patients with differentiated thyroid cancer (DTC) was studied on the background of thyroid hormone withdrawal (hypothyroidism) and the use of recombinant human thyroid­stimulating hormone — thyrogen (euthyroidism). Materials and methods. The immunological indices were determined in 2 groups of patients with DTC, who underwent radical surgery, in different conditions of the RIT. Group А (with thyroid hormone withdrawal) included 28 patients aged 24 to 60 years. Patients received 1 or 2 courses of RIT. Group В (with the use of thyrogen) comprised 23 patients aged 14 to 66 years. Patients of group В received 1 to 3 courses of RIT. Immunological examination was conducted the day before and 5 or 6 days after taking 131I with activity from 2180 to 6000 MBq. The control groups consisted of donors of the corresponding age and sex. The cytotoxic activity of NK cells against indicator target cells was determined using spectrophotometry. Metabolic activity of NF was evaluated in the standard nitroblue tetrazolium test. Results. After RIT, the activity of NK cells in patients with thyroid hormone withdrawal (group А) does not change significantly and remains at a low level, and the metabolic activity of NF significantly exceeds the baseline value. In patients, who received thyrogen (group В), the activity of NK cells after RIT is significantly increased relative to the baseline value, and the activity of NF does not undergo significant changes. The activity of NK cells in patients of group В is statistically significant (p < 0.001) higher, and the activity of NF is lower (p < 0.001) than the activity of these cells in patients of group А (withdrawal). Conclusions. A comparative analysis of the results showed that the activity of NK cells and the metabolic activity of NF in patients with DTC differ depending on the conditions of the RIT. In euthyroid patients with DTC after RIT, the activity of NK cells is greater, and NF is lower than that of these cells in hypothyroid patients. Preparation for RIT using recombinant human thyroid­stimulating hormone (thyrogen) positively influences the functional indices of innate immunity cells in patients with DTC treated with radioiodine.


thyroid cancer; radioiodine therapy; recombinant human thyroid-stimulating hormone; the activity of NK cells; metabolic activity of neutrophils


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