Essential microelement contents in the blood plasma of children with biologically inactive growth hormone syndrome
Background. The purpose was to increase the effectiveness of treatment in children and adolescents with biologically inactive growth hormone syndrome (BIGHS) based on studying the content of essential microelements (EM) in the blood plasma. Materials and methods. The study included 206 children and adolescents with different forms of short stature. Group with BIGHS consisted of 29 children (14.08 %): 21 boys (72.41 %) and 8 girls (27.59 %), with growth retardation from –2.0 to –4.6 SD. The average age of children was 9.01 ± 0.60 years. Diagnosis of BIGHS was confirmed by the presence of normal/elevated growth hormone parameters against the background of stimulation tests, a sharp decrease in the level of insulinlike growth factor1 (IGF1), and positive test for growth hormone sensitivity. The microelement status was assessed by determining the levels of EM (zinc, selenium, manganese, chromium, copper) in the blood plasma by Xray fluorescence spectrometry using the ElvaXmed spectrometer (Ukraine). Plasma levels of IGF1 were evaluated by immunoradiometric assay (IRMA) using standard IRMA IGF1 kits (Immunotech® kit, Czech Republic). Results. In the group of children with BIGHS, the levels of zinc and selenium were 0.57 ± 0.04 µg/ml and 0.05 ± 0.01 µg/ml, respectively, that is significantly lower than in the general group of children with short stature (p < 0.001 and p < 0.05, respectively). The average plasma levels of chromium and copper were lower than those in children with other forms of short stature and amounted to 0.040 ± 0.003 µg/ml and 0.79 ± 0.05 µg/ml, respectively, but were significantly lower than in the control group (р < 0.001). The average level of manganese in the blood plasma was 0.06 ± 0.02 µg/ml, which is somewhat lower than in the control group, but not significantly (p > 0.01). Conclusions. Patients with BIGHS were found to have potentially lower levels of zinc, selenium, chromium and copper in the blood plasma. Thus, in our opinion, it is advisable to include the determination of EM levels in a comprehensive examination of children with short stature. When detecting lower levels of EM, a combined treatment with recombinant growth hormone preparations and those containing appropriate EM is recommended that significantly increases the effectiveness of growthcorrection therapy.
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Pogorjelov MV, Bumejster VI, Tkach GF, et al. Makro- ta mikroelementy (obmin, patologija ta metody vyznachennja): monografija [Macro- and trace elements (exchange, pathology and methods of detection): monograph]. Sumy: SumDU; 2010. 147 p. (in Ukrainian).
Klatka M, Błażewicz A, Partyka M, Kołłątaj W, Zienkiewicz E, Kocjan R. Concentration of selected metals in whole blood, plasma, and urine in short stature and healthy children. Biol Trace Elem Res. 2015 Aug;166(2):142-8. doi: 10.1007/s12011-015-0262-2.
Rains TM, Mangian HF, Liang T, Cole AC, Beverly JL, Shay NF. Growth hormone-releasing factor affects macronutrient intake during the anabolic phase of zinc repletion: total hypothalamic growth hormone-releasing factor content and growth hormone-releasing factor immunoneutralization during zinc repletion. Nutr Neurosci. 2001;4(4):283-93.
Mayo-Wilson E, Junior JA, Imdad A, et al. Zinc supplementation for preventing mortality, morbidity, and growth failure in children aged 6 months to 12 years of age. Cochrane Database Syst Rev. 2014 May 15;(5):CD009384. doi: 10.1002/14651858.CD009384.pub2.
Kozarenko V. Defitsit tsinka u detei. Vliianie na tempy rosta i polovogo razvitiia [Zinc deficiency in children. Influence on the rate of growth and sexual development]. LAP Lambert Academic Publishing; 2011. 156 p. (in Russian).
Tronko MD, Polumbryk MO, Kovbasa VM, Kravchenko VI, Balion YH. The biological role of zinc on human body and necessity of sufficient level of its intake. Visn Nac Akad Nauk Ukr. 2013;(6):21-31. (in Ukrainian).
Bolshova OV, Pakhomova VH. Content of essential trace elements in the hair of children with short stature due to somatotropic insufficiency. ScienceRise: Medical Science. 2016;4(3):58-64. doi: 10.15587/2313-8416.2016.67690. (in Ukrainian).
Bolshova OV, Pakhomova VH. The content of insulin-like growth factor 1 and essential trace elements in the blood plasma of children with different forms of short stature. Mìžnarodnij endokrinologìčnij žurnal. 2016;(75):70-75. doi: 10.22141/2224-0722.214.171.1246.76639. (in Ukrainian).
Rising R, Scaglia JF, Cole C, Tverskaya R, Duro D, Lifshitz F. Exogenous recombinant human growth hormone effects during suboptimal energy and zinc intake. Nutr Metab (Lond). 2005 Apr 7;2(1):10. doi: 10.1186/1743-7075-2-10.
Aigner E, Theurl I, Haufe H, et al. Copper availability contributes to iron perturbations in human nonalcoholic fatty liver disease. Gastroenterology. 2008 Aug;135(2):680-8. doi: 10.1053/j.gastro.2008.04.007.
Maggio M, Ceda GP, Lauretani F, et al. Association of plasma selenium concentrations with total IGF-1 among older community-dwelling adults: the InCHIANTI study. Clin Nutr. 2010 Oct;29(5):674-7. doi: 10.1016/j.clnu.2010.03.012.
Karl JP, Alemany JA, Koenig C, et al. Diet, body composition, and physical fitness influences on IGF-I bioactivity in women. Growth Horm IGF Res. 2009 Dec;19(6):491-6. doi: 10.1016/j.ghir.2009.04.001.
Moreno-Reyes R, Egrise D, Nève J, Pasteels JL, Schoutens A. Selenium deficiency-induced growth retardation is associated with an impaired bone metabolism and osteopenia. J Bone Miner Res. 2001 Aug;16(8):1556-63. doi: 10.1359/jbmr.2001.16.8.1556.
Skalnaya MG, Skalny AV. Trace elements: the biological role and significance for medical practice. Communication 3. Manganese. Problems of Biological, Medical and Pharmaceutical Chemistry. 2015;(3):14-25. (in Russian).
Bol'shova OV, Pakhomova VH. The content of essential micronutrients in the body of children with a retardation in growth, depending on the degree of short growth. ScienceRise: Medical Science. 2016;6(23):50-56. doi: 10.15587/2519-4798.2016.72754. (in Ukrainian).
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