Apoptosis of blood lymphocytes in patients with autoimmune thyroiditis and its treatment

Main Article Content

M.I. Sheremet


Background. The purpose was to determine some links of pathogenesis of nodular goiter on the background of autoimmune thyroiditis (АТ) by studying the activity of lipid and protein peroxidation processes, apoptosis of peripheral blood lymphocytes and level of some cytokines before and after surgical intervention, to develop the effective me­thods of its correction. Materials and methods. Eighty women were exa­mined with nodular goiter on the background of АТ. Results. This article presents results of apoptosis detection in blood lymphocytes of patients with AT in euthyroid and hypothyroid state. Conclusions. Contents of CD95+, annexin V-positive (apoptotic) cells and tumor necrosis factor-α (TNF-α) in the blood were analyzed. It was shown that altered regulation of programmed lymphocyte death in autoimmune thyroiditis is manifested by increased content of TNF-α and CD95+ lymphocytes associated with decreased number of apoptotic cells in the blood. Meanwhile, this pathology is more expressed in patients with AT with euthyroid state than in thyroid hypofunction. Possible mechanisms of the revealed disturbances are discussed.

Article Details

How to Cite
Sheremet, M. “Apoptosis of Blood Lymphocytes in Patients With Autoimmune Thyroiditis and Its Treatment”. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY (Ukraine), vol. 14, no. 3, Apr. 2018, pp. 245-50, doi:10.22141/2224-0721.14.3.2018.136421.
Original Researches


Vecchiatti SM, Lin CJ, Capelozzi VL, Longatto-Filho A, Bisi H. Prevalence of thyroiditis and immunohistochemistry study searching for a morphologic consensus in morphology of autoimmune thyroiditis in a 4613 autopsies series. Appl Immunohistochem Mol Morphol. 2015;23(6):402-8. doi: 10.1097/PAI.0000000000000094.

Carney JA, Lyssikatos C, Seethala RR, et al. The Spectrum of Thyroid Gland Pathology in Carney Complex: The Importance of Follicular Carcinoma. Am J Surg Pathol. 2018;42(5):587-594. doi: 10.1097/PAS.0000000000000975.

Durante C, Grani G, Lamartina L, Filetti S, Mandel SJ, Cooper DS. The Diagnosis and Management of Thyroid Nodules: A Review. JAMA. 2018;319(9):914-924. doi: 10.1001/jama.2018.0898.

Pyzik A, Grywalska E, Matyjaszek-Matuszek B, Roliński J. Immune disorders in Hashimoto’s thyroiditis: what do we know so far? J Immunol Res. 2015;2015:979167. doi: 10.1155/2015/979167.

Kawashima ST, Tagami T, Nakao K, et al. Serum levels of IgG and IgG4 in Hashimoto thyroiditis. Endocrine. 2014;45(2):236-243. doi: 10.1007/s12020-013-9988-9.

Park S, Jeon MJ, Song E, et al. Clinical Features of Early and Late Postoperative Hypothyroidism After Lobectomy. J Clin Endocrinol Metab. 2017;102(4):1317-1324. doi: 10.1210/jc.2016-3597.

Xia Q, Dong S, Bian PD, Wang J, Li CJ. Effects of endocrine therapy on the prognosis of elderly patients after surgery for papillary thyroid carcinoma. Eur Arch Otorhinolaryngol. 2016;273(4):1037-43. doi: 10.1007/s00405-015-3564-2.

Ito M, Miyauchi A, Hisakado M, et al. Biochemical Markers Reflecting Thyroid Function in Athyreotic Patients on Levothyroxine Monotherapy. Thyroid. 2017;27(4):484-490. doi: 10.1089/thy.2016.0426.

Rojas-Villarraga A, Amaya-Amaya J, Rodriguez-Rodriguez A, Rubén D. Mantilla RD, Juan-Manuel Anaya JM. Introducing polyautoimmunity: secondary autoimmune diseases no longer exist. Autoimmune Dis. 2012;2012:254319. doi: 10.1155/2012/254319.

Figueroa-Vega N, Alfonso-Pérez M, Benedicto I, Sánchez-Madrid F, González-Amaro R, Marazuela M. Increased circulating pro-inflammatory cytokines and Th17 lymphocytes in Hashimoto’s thyroiditis. J Clin Endocrinol Metab. 2010;95(2):953 -62. doi: 62.10.1210/jc.2009-1719.

Ramos-Leví AM, Marazuela M. Pathogenesis of thyroid autoimmune disease: the role of cellular mechanisms. Endocrinol Nutr. 2016;63(8):421-9. doi: 10.1016/j.endonu.2016.04.003.

Morris GP, Brown NK, Kong YM. Naturally-existing CD4(+)CD25(+)Foxp3(+) regulatory T cells are required for tolerance to experimental autoimmune thyroiditis induced by either exogenous or endogenous autoantigen. J Autoimmun. 2009;33(1):68-76. doi: 10.1016/j.jaut.2009.03.010.

González-Amaro R, Marazuela M. T regulatory (Treg) and T helper 17 (Th17) lymphocytes in thyroid autoimmunity. Endocrine. 2016;52(1):30-8. doi: 10.1007/s12020-015-0759-7.

Kristensen B, Hegedüs L, Madsen HO. Altered balance between self-reactive T helper (Th)17 cells and Th10 cells and between full-length forkhead box protein 3 (FoxP3) and FoxP3 splice variants in Hashimoto’s thyroiditis. Clin Exp Immunol. 2015;180(1):58-69. doi: 10.1111/cei.12557. 

Weetman AP. The immunopathogenesis of chronic autoimmune thyroiditis one century after Hashimoto. Eur Thyroid J. 2013;1(4):243-50. doi: 10.1159/000343834.

Chen JQ, Papp G, Szodoray P, Zeher M. The role of microRNAs in the pathogenesis of autoimmune diseases. Autoimmun Rev. 2016;15(12):1171-80. doi: 10.1016/j.autrev.2016.09.003. 

Lepez T, Vandewoestyne M, Deforce D. Fetal microchimeric cells in autoimmune thyroid diseases: harmful, beneficial or innocent for the thyroid gland? Chimerism. 2013;4(4):111-8. doi: 10.4161/chim.25055.

Boelaert K, Newby PR, Simmonds MJ, et al. Prevalence and relative risk of other autoimmune diseases in subjects with autoimmune thyroid disease. Am J Med. 2010;123(2):183.e1-9. doi: 10.1016/j.amjmed.2009.06.030.