Changes in the convoluted tubules of the kidneys at the administration of NADP on the background of streptozotocin-induced diabetes mellitus in rats

M.I. Grytsiuk

Abstract


Background. The objective of the study was to investigate the changes in kidney tubular structures during nicotinamide adenine dinucleotide phosphate (NADP) administration on a background of streptozotocin-induced diabetes mellitus (DM) in rats. Materials and methods. The experiments were performed on male white rats, with modeled DM by administration of streptozotocin at a dose of 70 mg/kg. NADP was administered at a dose of 30 mg/kg. We studied the structure of the kidneys on the 11th, 21st and 31st day of the experiment. Results. In contrast to the classical methods of staining, early changes in various kidney structures were detected using a histochemical method based on the staining of histological sections with bromophenol blue by the method of Mikel Calvo. Conclusions. In general, the kidney structures showed a positive reaction to the administration of NADP, which may be determined by a reduction in the direct damaging effect of hyperglycemia, both due to a reduction in the number of glycosylated proteins and the direct positive effects on the endothelial cells.


Keywords


diabetes mellitus; nephropathy; streptozotocin; convoluted tubules

References


Skrobons'ka NA, Cymbal TS. Diabetic nephropathy: some unconventional causes of pathogenesis, basic directions of diagnosis and treatment (literature review and own data). Simejna medycyna. 2011;4:18-22. (in Ukrainian).

Alicic RZ, Rooney MT, Tuttle KR. Diabetic Kidney Disease: Challenges, Progress, and Possibilities. Clin J Am Soc Nephrol. 2017 Dec 7;12(12):2032-2045. doi: 10.2215/CJN.11491116.

Urushihara M, Kagami S. Role of the intrarenal renin-angiotensin system in the progression of renal disease. Pediatr Nephrol. 2017;9:1471-1479. doi: 10.1007/s00467-016-3449-7.

Jefimov AS, Skrobons'ka NA, Cymbal TS. Pathogenetic markers in the development of diabetic nephropathy in 1 type diabetes mellitus patients, their importance in early diagnosis and treatment optimization. Endokrynologia. 2014;19(4):295-296. (in Ukrainian).

Mora-Fernández C, Domínguez-Pimentel V, de Fuentes MM, Górriz JL, Martínez-Castelao A, Navarro-González JF. Diabetic kidney disease: from physiology to therapeutics. J Physiol. 2014 Sep 15;592(18):3997-4012. doi: 10.1113/jphysiol.2014.272328.

Yang GK, Maahs DM, Perkins BA, Cherney DZ. Renal hyperfiltration and systemic blood pressure in patients with uncomplicated type 1 diabetes mellitus. PLoS One. 2013 Jul 4;8(7):e68908. doi: 10.1371/journal.pone.0068908.

Zeni L, Norden AGW, Cancarini G, Unwin RJ. A more tubulocentric view of diabetic kidney disease. J Nephrol. 2017 Dec;30(6):701-717. doi: 10.1007/s40620-017-0423-9.

Srivastava T, Thiagarajan G, Alon US, et al. Role of biomechanical forces in hyperfiltration-mediated glomerular injury in congenital anomalies of the kidney and urinary tract. Nephrol Dial Transplant. 2017 May 1;32(5):759-765. doi: 10.1093/ndt/gfw430.

Zhang C, Meng Y, Liu Q, et al. Injury to the endothelial surface layer induces glomerular hyperfiltration rats with early-stage diabetes. J Diabetes Res. 2014; 2014: 953740. doi: 10.1155/2014/953740.

Olinyk OJ, Fediv OI, Davydenko IS, Ushakov AV. The oxidant-antyoxydant homeostasis and oxidative modifi cation of proteins in patients with gastric and duodenal ulcers, combined with the diabetes mellitus, in the dynamics of treatment. Patologia. 2010;7(3):87-90. (in Ukrainian).

Bequer L, Gómez T, Molina JL, Artiles D, Bermúdez R, Clapés S. Streptozotocin diabetogenic action in an experimental neonatal induction model. Biomedica. 2016 Jun 3;36(2):230-8. doi: 10.7705/biomedica.v36i2.2686.

Kolesnyk YuM, Ivanenko TV, Abramov AV, Kuzo NV. Current methods of the modeling of experimental diabetes mellitus type 2: a literature review. Pathologia. 2016;1(36):10-14. doi: 10.14739/2310-1237.2016.1.72365.

Davydenko IS, Davydenko OM. Histochemical peculiarities of protein oxidative modification in the cells of the renal glomerule in acute postinfective glomerulonephritis. Buk Med Herald. 2012;16(3 part 2):106-108. (in Ukrainian).

Davydenko IS. Measures of standardization of the histochemical method on oxidizing modification of proteins. Ukrai'ns'kyj medychnyj al'manah. 2013;16 Suppl 3:180-181. (in Ukrainian).




DOI: https://doi.org/10.22141/2224-0721.13.8.2017.119282

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