Immune histochemical study of apoptosis marker expression and proliferation in tissues at follicular neoplasia of the thyroid gland
Background. The purpose was to determine the expression of immune histochemical markers of apoptosis, р53 and bcl-2, and proliferation marker Ki-67 in follicular thyroid neoplasia. Materials and methods. Numerous immune histochemical markers are proposed as criteria for the differential diagnosis of follicular thyroid neoplasia, the most useful of which are Ki-67 proliferation marker and p53 and bcl-2 apoptosis markers. We have conducted immune histochemical study of the surgical material of 50 patients operated in the clinic of State Institution “V. Danilevsky Institute of Endocrine Pathology Problems of the NAMS of Ukraine” for benign (25 adenoid tumors) and malignant (25 carcinoma) follicular neoplasms. Results. The obtained data shows that follicular adenomas with solid and fetal-embryonic structure have a high level of correlation with follicular thyroid carcinomas in terms of proliferative activity and dysfunction of apoptosis mechanisms, which, probably, presumes the greatest potential for malignancy and further metastasis. Conclusions. High level of р53 expression and stably high level of bcl-2 expression in the cells of follicle thyroid cancer specifies deep violations of apoptotic processes in development of follicular thyroid neoplasia.
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