DOI: https://doi.org/10.22141/2224-0721.13.6.2017.112881

Endothelial dysfunction and morphofunctional properties of erythrocytes in non-alcoholic steathohepatitis combined with chronic obstructive pulmonary disease in patients with obesity

T.P. Cyntar, O.I. Fediv, G.Ya. Stupnytska, S.V. Glugovska

Abstract


Background. The investigation of violation of the functional state of the endothelium and morphofunctional properties of erythrocytes in non-alcoholic steatohepatitis (NASH) combined with chronic obstructive pulmonary disease (COPD) in patients with obesity was the purpose of our study. Materials and methods. We have examined 30 patients with NASH on the background of degree I obesity (group 1), 30 patients with COPD and normal body weight (group 2), 60 patients with NASH in combination with COPD (group 3) and 20 apparently healthy persons. The functional state of the endothelium was studied by the content of stable metabolites of nitrogen monoxide (nitrites/nitrates), endothelin-1 (ET-1), and the number of circulating excised endothelial cells (CEEC) with soluble vascular cell adhesion molecule-1 (sVCAM-1) content in the blood. The erythrocyte deformity index (EDI), the relative viscosity of the erythrocytic suspension (RVES) and the percentage of peroxide hemolysis of erythrocytes (PHE) were also determined. Results. The conducted study showed that the course of NASH in its combination with COPD in patients with degree I obesity is accompanied by an increase in the level of ET-1 (by 5.8 times), sVCAM-1 (5.6-fold), CEEC (2.1-fold), RVES (by 65 %) and the percentage of PHE (2.1-fold) while simultaneously reducing the content of stable metabolites of nitrogen monoxide (nitrites/nitrates) (2.1-fold) and EDI (by 36.2 %) compared to those in apparently healthy subjects. Conclusions. With a combined course of NASH and COPD in patients with degree I obesity, endothelial dysfunction develops, which is characterized by elevated levels of ET-1, sVCAM in the blood serum and CEEC, with the maximum decrease in the content of nitrites/nitrates in the blood. One of the factors leading to the aggravation of microcirculatory disorders in NASH combined with COPD is the changes in the morphofunctional state of erythrocytes (reduction of the EDI with simultaneous increase in RVES and percentage of PHE).

Keywords


endothelial dysfunction; erythrocyte; non-alcoholic steatohepatitis; obesity; chronic obstructive pulmonary disease

References


Persico M, Masarone M, Damato A, et al. Non alcoholic fatty liver disease and eNOS dysfunction in humans. BMC Gastroenterol. 2017;17(1):35. doi: 10.1186/s12876-017-0592-y.

Federico A, Dallio M, Masarone M, Persico M, Loguercio C. The epidemiology of non-alcoholic fatty liver disease and its connection with cardiovascular disease: role of endothelial dysfunction. Eur Rev Med Pharmacol Sci. 2016;20(22):4731-4741. PMID: 27906428.

Fan Y, Wei F, Zhou Y, Zhang H. Association of non-alcoholic fatty liver disease with impaired endothelial function by flow-mediated dilation: A meta-analysis. Hepatol Res. 2016;46(3):165-73. doi: 10.1111/hepr.12554.

Ambrosino P, Lupoli R, Iervolino S, et al. Clinical assessment of endothelial function in patients with chronic obstructive pulmonary disease: a systematic review with meta-analysis. Intern Emerg Med. 2017. doi: 10.1007/s11739-017-1690-0.

Green CE, Turner AM. The role of the endothelium in asthma and chronic obstructive pulmonary disease (COPD). Respir Res. 2017;18(1):20. doi: 10.1186/s12931-017-0505-1.

Kapustnik V, Istomina О. Endothelial Dysfunction In Patients With Chronic Obsrtructive Pulmonary Disease With Concomitant Hypertension. Georgian Med News. 2016;256-257:29-33. PMID: 27661272.

Gonzalez-Paredes FJ, Hernández Mesa G, Morales Arraez D, et al. Contribution of Cyclooxygenase End Products and Oxidative Stress to Intrahepatic Endothelial Dysfunction in Early Non-Alcoholic Fatty Liver Disease. PLoS One. 2016;11(5):e0156650. doi: 10.1371/journal.pone.0156650.

Pasarín M, La Mura V, Gracia-Sancho J, et al. Sinusoidal endothelial dysfunction precedes inflammation and fibrosis in a model of NAFLD. PLoS One. 2012;7(4):e32785. doi: 10.1371/journal.pone.0032785.

Jiang Y, Wang X, Hu D. Mitochondrial alterations during oxidative stress in chronic obstructive pulmonary disease. Int J Chron Obstruct Pulmon Dis. 2017;12:1153-1162. doi: 10.2147/COPD.S130168.

Polverino F, Laucho-Contreras ME, Petersen H, et al. A Pilot Study Linking Endothelial Injury in Lungs and Kidneys in Chronic Obstructive Pulmonary Disease. Am J Respir Crit Care Med. 2017 Jun 1;195(11):1464-1476. doi: 10.1164/rccm.201609-1765OC.

Lebed' KN. Markers of systemic inflammation in patients with chronic obstructive pulmonary disease associated with non-alcoholic steatohepatitis. Problemy ekolohichnoi ta medychnoi henetyky i klinichnoi imunolohii. 2012;3:200-204. (in Russian).

Lebed' KN. Peroxide lipid oxidation and state of the system of antioxidant protection in patients with chronic obstructive pulmonary disease combined with nonalcoholic steatohepatitis. Ukrainskyi medychnyi almanakh. 2013;16(1):138-141. (in Ukrainian).

Byrne CD, Targher G. NAFLD: a multisystem disease. J Hepatol. 2015 Apr;62(1 Suppl):S47-64. doi: 10.1016/j.jhep.2014.12.012.

Petta S, Valenti L, Bugianesi E, et al. A “systems medicine” approach to the study of non-alcoholic fatty liver disease. Dig Liver Dis. 2016 Mar;48(3):333-42. doi: 10.1016/j.dld.2015.10.027.

Lonardo A, Nascimbeni F, Ponz de Leon M. Nonalcoholic fatty liver disease and COPD: is it time to cross the diaphragm? Eur Respir J. 2017;49(6). pii: 1700546. doi: 10.1183/13993003.00546-2017.

Viglino D, Jullian-Desayes I, Minoves M, et al. Nonalcoholic fatty liver disease in chronic obstructive pulmonary disease. Eur Respir J. 2017;49(6). pii: 1601923. doi: 10.1183/13993003.01923-2016.

Minakata Y, Ueda H, Akamatsu K, et al. High COPD prevalence in patients with liver disease. Intern Med. 2010;49:2687-91. PMID: 21173543.

Jung DH, Shim JY, Lee HR, Moon BS, Park BJ, Lee YJ. Relationship between non-alcoholic fatty liver disease and pulmonary function. Intern Med J. 2012 May;42(5):541-6. doi: 10.1111/j.1445-5994.2011.02644.x.

Mapel DW, Marton JP. Prevalence of renal and hepatobiliary disease, laboratory abnormalities, and potentially toxic medication exposures among persons with COPD. Int J Chron Obstruct Pulmon Dis. 2013;8:127-34. doi: 10.2147/COPD.S40123.

Peng TC, Kao TW, Wu LW, et al. Association between pulmonary function and nonalcoholic fatty liver disease in the NHANES III study. Medicine (Baltimore). 2015 May;94(21):e907. doi: 10.1097/MD.0000000000000907.

Qin L, Zhang W, Yang Z, et al. Impaired lung function is associated with non-alcoholic fatty liver disease independently of metabolic syndrome features in middle-aged and elderly Chinese. BMC Endocr Disord. 2017 Mar 22;17(1):18. doi: 10.1186/s12902-017-0168-4.

Lefere S, Van de Velde F, Devisscher L, et al. Serum vascular cell adhesion molecule-1 predicts significant liver fibrosis in non-alcoholic fatty liver disease. Int J Obes (Lond). 2017;41(8):1207-1213. doi: 10.1038/ijo.2017.102.

Federico A, Dallio M, Masarone M, Persico M, Loguercio C. The epidemiology of non-alcoholic fatty liver disease and its connection with cardiovascular disease: role of endothelial dysfunction. Eur Rev Med Pharmacol Sci. 2016;20(22):4731-41. PMID: 27906428.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

© "Publishing House "Zaslavsky", 1997-2018

 

   Seo анализ сайта