Taxonomic structure and population level of colon microbial contents in white rats with experimental thyrotoxicosis

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L.I. Sydorchuk


Background. Production of numerous biologically active compounds and their metabolites by intestinal microflora, interaction with the immune and other systems is of great importance while studying its changes in various diseases, one of which is thyrotoxicosis. So, the purpose of this study was to determine the severity of intestine microbioma disorder in white rats with experimental thyrotoxicosis (ET). Materials and methods. Studies were carried out on 25 mature male white rats (15 — control group, 10 — research group). ET was simulated by intragastric administration of L-thyroxine for 14 days. Under sterile conditions a laparotomy was performed, a section (2–3 cm) of the large intestine with its contents was taken. Sterile 0.9% NaCl solution was added to the content. Series of ten-fold dilutions with a concentration of the initial mixture of 10–2 to 10–11 was prepared. From each test tube 0.01 ml was seeded on solid nutrient media with subsequent isolation and identification of microbes according to morphological, tinctorial, cultural and biochemical properties. Results. The results of the study demonstrated that in ET animals the main microbioma is represented by bacteria Bifidobacterium, Lactobacillus, Bacteroides, and also opportunistic enterobacteria (Escherichia, Proteus, Klebsiella), peptococcus, staphylococci and clostridia. This is accompanied by the elimination of Peptostreptococcus, Enterococcus from bacterial biotope and the contamination of K. oxytoca and staphylococci. There was a pronounced deficit of bifidobacteria by 42.81 %, lactobacillus by 22.57 %, normal intestinal bacillus by 16.48 %. By the population level, the coefficient of quantitative dominance and the significance factor, the leading place is occupied by bacteroids, role of which is increased by 21.72 %, and lactobacillus role decreases by 39.31 %, bifidobacteria decreases by 51.48 % and E. coli decreases by 57.49 %. In this case, the role of peptococcus 3.37-fold increases, clostridia by 4.53, and by 73.93 % by the number of proteus. Conclusions. Under conditions of ET, there is an elimination of Bifidobacterium, Lactobacillus, Peptostreptococcus, Enterococcus and contamination of the biotope with conditionally pathogenic enterobacteria (Proteus, Klebsiella) and staphylococci. Deficiency of bifidobacteria and lactobacilli leads to changes in taxonomic structure and formation of dysbiosis of II and III stage.

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Sydorchuk, L. “Taxonomic Structure and Population Level of Colon Microbial Contents in White Rats With Experimental Thyrotoxicosis”. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY (Ukraine), vol. 13, no. 5, Oct. 2017, pp. 380-5, doi:10.22141/2224-0721.13.5.2017.110029.
Experimental Endocrinology


Biedermann L, Rogler G. The intestinal microbiota: its role in health and disease. Eur J Pediatr. 2015 Feb;174(2):151-67. doi: 10.1007/s00431-014-2476-2.

Tojo R, Suárez A, Clemente MG, at al. Intestinal microbiota in health and disease: role of bifidobacteria in gut homeostasis. World J Gastroenterol. 2014 Nov 7; 20(41): 15163-76. doi: 10.3748/wjg.v20.i41.15163.

van Baarlen P,Wells JM, Kleerebezem M. Regulation of intestinal homeostasis and immunity with probiotic lactobacilli. Trends Immunol. 2013 May;34(5):208-15. doi: 10.1016/

Goulet O. Potential role of the intestinal microbiota in programming health and disease. Nutr Rev. 2015 Aug;73(Suppl 1):32-40. doi: 10.1093/nutrit/nuv039.

Iqbal S,Quigley EM. Progress in Our Understanding of the Gut Microbiome: Implications for the Clinician. Curr Gastroenterol Rep. 2016 Sep;18(9):49. doi: 10.1007/s11894-016-0524-y.

Lynch SV, Pedersen O. The Human Intestinal Microbiome in Health and Disease. N Engl J Med. 2016 Dec 15;375(24):2369-79. doi: 10.1056/NEJMra1600266.

Kim D, Yoo SA, Kim WU. Gut microbiota in autoimmunity: potential for clinical applications. Arch Pharm Res. 2016 Nov;39(11):1565-76. doi: 10.1007/s12272-016-0796-7.

Ho JT, Chan GC, Li JC. Systemic effects of gut microbiota and its relationship with disease and modulation. BMC Immunol. 2015 Mar 26;16:21. doi: 10.1186/s12865-015-0083-2.

Covelli D, Ludgate M. The thyroid, the eyes and the gut: a possible connection. J Endocrinol Invest. 2017 Jun;40(6):567-76. doi: 10.1007/s40618-016-0594-6.

Köhling HL, Plummer SF, Marchesi JR, Davidge KS, Ludgate M. The microbiota and autoimmunity: Their role in thyroid autoimmune diseases. Clin Immunol. 2017 Jul 6;183:63-74. doi: 10.1016/j.clim.2017.07.001.

Kunc M, Gabrych A, Witkowski JM. Microbiome impact on metabolism and function of sex, thyroid, growth and parathyroid hormones. Acta Biochim Pol. 2016;63(2):189-201. doi: 10.18388/abp.2015_1093.

Virili C, Centanni M. "With a little help from my friends" - The role of microbiota in thyroid hormone metabolism and enterohepatic recycling. Mol Cell Endocrinol. 2017 Feb 4. pii: S0303-7207(17)30075-8. doi: 10.1016/j.mce.2017.01.053.

Neuman H, Debelius JW, Knight R, Koren O. Microbial endocrinology: the interplay between the microbiota and the endocrine system. FEMS Microbiol Rev. 2015 Jul;39(4):509-21. doi: 10.1093/femsre/fuu010.

Evans JM, Morris LS, Marchesi JR. The gut microbiome: the role of a virtual organ in the endocrinology of the host. J Endocrinol. 2013 Aug 28;218(3):R37-47. doi: 10.1530/JOE-13-0131.

Tomasello G, Tralongo P, Amoroso F, et al. Dysmicrobism, inflammatory bowel disease and thyroiditis: analysis of the literature. J Biol Regul Homeost Agents. 2015 Apr-Jun;29(2):265-72. PMID: 26122213.

Sydorchuk LІ. Сolonization resistance of the mucous coat of the small intestinal distal portion of splenectomized albino rats. Buk Med Herald. 2011;15(57):150-4. (in Ukrainian).

Zhou L, Li X, Ahmed A, et al. Gut microbe analysis between hyperthyroid and healthy individuals. Curr Microbiol. 2014 Nov;69(5):675-80. doi: 10.1007/s00284-014-0640-6.

Mazur OA, Pashkovskaia NV, Levitskaia SA, et al. Quantitative and Qualitative Composition of Colonic Content Microbiota in Diabetes Mellitus Type 1 Patients with Concomitant Chronic Purulent Maxillary Sinusitis. Mezhdunarodnyi Endokrinologicheskii Zhurnal. 2015;2(66):37-42 (in Ukrainian).

Hansen CH, Yurkovetskiy LA, Chervonsky AV. Cutting edge: commensal microbiota has disparate effects on manifestations of polyglandular autoimmune inflammation. J Immunol. 2016 Aug 1;197(3):701-5. doi: 10.4049/jimmunol.1502465.