Development of experimental alloxan model of diabetes mellitus
Background. One of the main causes that lead to the disability of diabetic patients is diabetic retinopathy (DR). The relevance of the problem of DR necessitates the development of optimal experimental models on experimental animals to find effective ways of correcting this pathology. The purpose of our work was to develop an experimental alloxan model of type 1 diabetes mellitus (DM) for the study of DR, which would not result in the lethal outcome of experimental animals under the action of alloxan; histological examination of changes in the tissues of the eyeball in the reproduction of the DM model for the selection of new effective methods for the metabolic treatment of DR in the early stages. Materials and methods. The experiment was carried out on white outbred Wistar rats weighing 180–200 g. The first group consisted of 20 animals that were not subjected to any influence, served as a control; second group — 30 animals, in which DM was modeled by administration of alloxan and fructose. Results. When modeling DR, vessel changes in the form of wall fibrosis, edema of the endothelium and vasospasm were found. There was also a decrease in the amount of pigment granules, dystrophic changes in the cells of the ganglionic layer and a layer of retinal rods and cones, which coincides with the descriptions of damage to the coats of the eyeball in patients with DM. Conclusions. In our studies, we have calculated the optimal dose of alloxan administration, which does not lead to the death of rats (the lethality of rats was absent) and is an effective model not only of DM in general, but also of DR.
Full Text:PDF (Українська)
World Health Organization. Diabetes. WHO Newsletter. November 2016 [Electronic resource]. (In Russian). Available from: http://www.who.int/mediacentre/factsheets/fs312/ru/
Pan'kiv V.I. Diabetes mellitus: definition, classification, epidemiology, risk factors. Mezhdunarodnyi Endokrinologicheskii Zhurnal. 2013;7(55):95-104. (In Ukrainian). doi: 10.22141/2224-07126.96.36.1993.84685.
Zelins'ka NB, Horoshaja OO, Starynec’ NA. The frequency of diabetic retinopathy in children and adolescents with diabetes according to screening in the regions of Ukraine. Klinichna endokrynologija ta endokrynna hirurgija. 2005;4(13):27-31. (In Ukrainian).
Vergouwe Y, Soedamah-Muthu S, Zgibor J, et al. Progression to microalbuminuria in type 1 diabetes: development and validation of prediction rule. Diabetologia. 2010;53:254-62. doi: 10.1007/s00125-009-1585-3.
Pal'chikova NA, Kuznecova NV, Kuz'mynova OI, Seljatyckaya VG. Hormonal and biochemical features of alloxan and streptozotocin-induced models of experimental diabetes. Sibirskij nauchnyj medicinskij zhurnal. 2013;33(6):18-24. (In Russian).
Baranov VG, Sokoloverova IM, Gasparjan EG. et al. Eksperimental'nyj saharnyj diabet. Rol' v klinicheskoj diabetologii [Experimental diabetes mellitus. Role in clinical diabetes]. Leningrad: Nauka; 1983. 240 p. (In Russian).
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.
© "Publishing House "Zaslavsky", 1997-2017