Influence of preparation of BNO 1030 on pro-/antioxidant status of the blood in experimental type 1 diabetes mellitus

Yu.V. Gavrylenko, А.А. Layko, T.M. Kuchmirovska, M.M. Gudzyk

Abstract


Background. Among the mechanisms that lead to the development and deterioration of the diabetes mellitus type 1 (DM1) and its complications, an important role belongs to the intensification of oxidative stress. In the development of many late complications of DM1, in particular, micro- and macroangiopathies, nephropathy and others, oxidative stress is one of the main pathogenetic mechanisms. Objective. To evaluate the effect of phytopreparation BNO 1030 on pro/antioxidant status of the blood in experimental type 1 diabetes mellitus. Materials and methods. The studies were performed on intact male Wistar rats, in which experimental DM1 was modeled. The production of reactive oxygen species in the blood was measured using 2’,7’-dichlorofluorescein diacetate. The content of living, apoptotic and necrotic leukocytes was determined using annexin V conjugated with GFP (green fluorescent protein) and PI (propidium iodide). Results. Against the background of a decrease in body weight and an increase in the concentration of glucose in the blood of diabetic animals, an enhanced formation of reactive oxygen species was established in comparison with the control group of animals that indicates an intensification of the oxidative stress. Under these conditions, the activity of superoxide dismutase decreased, which indicates a violation of regulation between pro- and antioxidant processes. Conclusions. Administration of BNO 1030 to animals with experimental DM1 resulted in the slight intensification of the production of reactive oxygen species both in the control and in the diabetic group, but its effect increased the viability of immune cells, preventing their death. The use of BNO 1030 resulted in an increase of the activity of superoxide dismutase by 10 % compared to the group of diabetic animals.


Keywords


BNO 1030; white blood cells; reactive oxygen species; cell viability; experimental type 1 diabetes mellitus

References


Chattopadhyay S, Ramanathan M, Das J, Bhattacharya SK. Animal models in experimental diabetes mellitus. Indian Journal of Experimental Biology. 1997;35(11):1141-5. PMID: 9567740.

Ewing JF, Janero DR. Microplate superoxide dismutase assay employing a nonenzymatic superoxide generator. Anal Biochem. 1995;232(2):243-248. PMID: 8747482. doi: 10.1006/abio.1995.0014.

Kashiwagi A, Asahina T, Nishio Y. et al. Glycation, oxidative stress, and scavenger activity. Glucose metabolism and radical scavenger dysfunction in endothelial cells. Diabetes 1996;45:84-86. PMID: 8674901.

Muller J, Hoepelman AIM, Rutten GEHM Increased Risk of Common Infections in Patients with Type 1 and Type 2 Diabetes Mellitus. Clinical Infectious Diseases. 2005;41(3):281-8. PMID: 16007521. doi: 10.1086/431587.

Prentki M, Nolan CJ. Islet β cell failure in type 2 diabetes. J Inv. 2006;116(7):1802-1812. PMID: 16823478. doi: 10.1172/JCI29103.

Poitout V, Robertson RP. Minireview: secondary β-cell failure in type 2 diabetes – a convergence of glucotoxicity and lipotoxicity. Endocrinology 2002;143(2):339-342. PMID: 11796484. doi: 10.1210/endo.143.2.8623.

Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44-84. PMID: 16978905. doi: 10.1016/j.biocel.2006.07.001.

Yang H, Jin X, Lam CWK, Yan SK. Oxidative stress and diabetes mellitus. Clin Chem Lab Med. 2011;49(11):1773-82. PMID: 21810068. doi: 10.1515/CCLM.2011.250.

Zheng W, Huang LZ, Zhao L, Wang B, Xu HB, Wang GY, Wang ZL, Zhou H. Superoxide dismutase activity and malondialdehyde level in plasma and morphological evaluation of acute severe hemorrhagic shock in rats. Am J Emerg Med. 2008;26(1):54-58. PMID: 18082781. doi: 10.1016/j.ajem.2007.02.007.

Yasunari K, Maeda K, Nakamura M, Yoshikawa J. Oxidative stress in leukocytes is a possible link between Blood pressure, Blood Glucose, and C-Reacting Protein. Hypertension. 2002;39:777-80. doi: 10.1161/hy0302.104670.

Berger T. Tolerability and efficacy of a herbal combination preparation in children and adolescents with recurrent infections of the upper respiratory tract. MMW Fortschr Med. 2008;150(2):85-90. (In German). PMID: 18712128.

Stavniichuk R, Drel VR, Shevalye H, Maksimchyk Y, Kuchmerovska TM, Nadler JL, Obrosova IG. Baicalein alleviates diabetic peripheral neuropathy through inhibition of oxidative-nitrosative stress and p38 MAPK activation. Exp Neurol. 2011;230(1):106-13. PMID: 21515260. doi: 10.1016/j.expneurol.2011.04.002.




DOI: https://doi.org/10.22141/2224-0721.13.3.2017.104116

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

© "Publishing House "Zaslavsky", 1997-2017

 

 Яндекс.МетрикаSeo анализ сайта Рейтинг@Mail.ru